PepTest Bulk is MAN Sports’ most recent innovation which essentially combines the focus/energy enhancement of a pre-workout with the test-boosting properties of D-Aspartic Acid…FIND IT HERE
Acetyl-L-Carnitine (ALCAR) has been pretty extensively researched for a variety of purposes relating to physical performance and cognition but, in the context of PepTest Bulk, we’ll focus on the cognitive effects.
A 2001 study from the “Journal of Neuroscience Research” found that ALCAR supplementation was able to improve indications of learning in older mice at a dose that would translate into a 200lb human consuming about 1g.
In humans, ALCAR has been demonstrated to reduce the severity of cognitive decline in elderly dementia patients, as well as in older subjects with general cognitive decline (not any particular cognitive disease).
Unfortunately, studies regarding the effects of ALCAR in healthy individuals are limited, and at this time there is insufficient evidence to suggest it is particularly effective as a nootropic. That said, clearly ALCAR possess pro-cognitive properties so it makes sense in the context of PepTest Bulk.
Caffeine is a well-established ergogenic aid, oral consumption of which triggers the release of Catcholamines (Noradrenaline, Dopamine, Adrenaline, etc.), generally inducing a state of increased alertness, focus, and perceived energy.
Additionally, Caffeine can enhance calcium-ion release in muscle tissue, which directly increases muscle contraction force. Rather than discuss dozens of studies, we’ll leave it at this: Caffeine is an extremely effective ergogenic aid, though tolerance build-up is certainly an issue to keep in mind.
MAN lists the amount of Caffeine Anhydrous in PepTest Bulk at 200mg per serving, enough to make the average individual feel noticeably more alert and focused, especially in combination with several of the other ingredients in PepTest Bulk.
Tyrosine is a non-essential amino acid (the body can produce it from Phenylalanine) which serves a precursor to Dopamine (by first being converted into L-Dopa) and Noradrenaline.
Because of this relationship, Tyrosine is alleged to increase levels of these neurotransmitters, which would theoretically lead to performance enhancement. However, research has demonstrated that Tyrosine cannot outright raise Dopamine or Noradrenaline levels upon ingestion, though it can help maintain optimal levels when depletion might otherwise occur.
Upon ingestion, Tyrosine forms substrate pool, which can then be drawn from when an acute stressor (exercise, cold exposure, etc.) causes a temporary depletion of Dopamine/Noradrenaline. For this reason, Tyrosine can be useful for maintaining cognitive function during stressful activity.
PepTest Bulk contains an undisclosed amount of Tyrosine, but we’d estimate no more than 200mg or so.
GREEN TEA EXTRACT (EGCG)
Green Tea contains a catechin known as Epigallocatechin gallate (EGCG) and is generally standardized to a specific concentration. EGCG is a moderately potent anti-oxidant, but can also inhibit Catechol-O-Methyl Transferase (COMT), the enzyme responsible for the degradation of catecholamine neurotransmitters (Noradrenaline, Dopamine, etc.).
By inhibiting COMT, EGCG can potoentiate the effects of other stimulants such as Caffeine and Higenamine, and functions similarly to Hordenine as well. In the context of PepTest Bulk, EGCG may indeed be effective for enhancing focus and energy.
NELUMBO NUCIFERA (HIGENAMINE)
Nelumbo Nucifera (NN), sometimes referred to as Indian Lotus, is a flowering plant which has been shown to have anti-obesity effects in mice. The lipolytic effects of Nelumbo Nucifera are primarily attributed to the Higenamine, although there are other compounds which may potentiate these effects. Higenamine is a Beta(2) Adrenergic Agonist (same mechanism as Ephedra), meaning it stimulates the Beta(2) Adrenergic Receptors which initiate fat-burning.
MAN doesn’t tell us exactly how much Higenamine is present in PepTest Bulk, but no optimal dose has been established for humans anyway, so it doesn’t really matter.
HORDEUM VULGARE (HORDENINE)
Hordenine is included in many pre-workout supplements and fat-burners because of its ability to amplify the effects of Caffeine. In vitro and animal studies indicate that its primary mechanism of action is via Momoamine Oxidase inhibition, with oral doses being shown to augment Noradrenaline-induced muscle contraction while not directly inducing contractions itself.
So, rather than acting as a stand-alone stimulant, Hordenine can amplify/extend the effects of other stimulants by blocking the reuptake of Noradrenaline (and other Monoamines).
MAN Sports does not disclose the amount of Hordenine present in PepTest Bulk but it doesn’t take much to be effective. Certainly no reason to suspect a less-than-effective dose here.
MAN Sports makes no mention of Cocoa extract in any of the PepTest Bulk literature we’ve reviewed, so we’re not really sure what the proposed benefit is in this instance. Generally speaking, Cocoa is standardized for xanthines such as Theobromine and Theophylline.
Sceletium tortuosum, also known as Kanna, is an herb mostly indigenous to South Africa which has a long history of use as a mood elevator/anxiolytic. Kanna contains two types of alkaloids, mesembrine tortuosamine, which are alleged to convey the psychoactive effects.
A 2011 study published in the “Journal of Ethnopharmacology” found that Sceletium tortuosum extract had limited anxiolytic effects in rats subjected to psychological stress (in the form of restraint). While no mechanism of action has been established, the results of the study indicated that the herb does not act as a serotonin reuptake inhibitor (SSRI).
MAN fans should be familiar with Sceletium tortuosum, as the brand has used it in NOOPump as well. It’s unclear what dose is present in PepTest Bulk but, given that no “optimal dose” exists, it doesn’t really matter anyway.
Coleus Forskohlii is generally standardized for the active component, Forskolin (sometimes spelled Forskohlin), which has implications for both weight-loss and increasing Testosterone. Forskohlin works by increasing Cyclic Adenosine Monophosphat (cAMP), a signaling molecule which, when elevated, can induce a wide variety of physiological changes including increased Tesetosterone, increased lean body mass, and decreased fat mass.
A 2005 study, published in “Obesity Research”, found that Forskohlin (25mg/day for 12 weeks) increased Testosterone (total test increased 16%, free test increased 3.4%) and favorably influenced body composition in obese men.
Though the effects of Forskohlin on Testosterone levels in humans have not been studied extensively, Forskohlin is quite reliable at increasing cAMP, and cAMP is quite reliable at increasing Testosterone. So ultimately, Forskohlin possesses a sound mechanism by which it can positively influence Testosterone levels in humans.
MAN Sports does not list the exact dose of Coleus Forskohlii present in PepTest Bulk, but given that this particular form is standardized for 95% Forskohlin, it would only take 25-30mg to be effective for boosting Testosterone.
D-Aspartic Acid has become extremely popular as a Test-booster in recent years, with research suggesting it is indeed effective, at least in the short term.
A 2012 study from “Advances in Sexual Medicine”, the subjects of which were infertile men (initially low Testosterone) found that 2.66g of D-Aspartic Acid was able to significantly increase Testosterone levels when measured after 90 days of supplementation. These results were in-line with those of an earlier study from 2009 in which D-Aspartic Acid supplementation raised Testosterone by 42% after 12 days in healthy men (initially normal Testosterone).
However, a 2013 study published in “Nutrition Research” found that athletes who supplemented with D-Aspartic Acid for 28 days showed no difference in testosterone levels.
The researchers in the failed study noted abnormally high levels of D-Aspartate Oxidase, the enzyme which degrades D-Aspartic Acid, indicating that prolonged supplementation in individuals with healthy Testosterone levels may cause “negative feedback”.
PepTest Bulk contains a clinically effective 3120mg dose of D-Aspartic Acid per serving.
In the past few years, Agmatine has gone from a rare ingredient to pre-workout staple, though it remains seriously under-researched relative to other popular pre-workout ingredients. Agmatine has been demonstrated to up-regulate Endothelial Nitric Oxide (eNOS), sometimes referred to as the “good” NOS, while inhibiting the other NOS enzymes (the “bad” NOS) in vitro, but human studies are non-existent.
While Agmatine is commonly touted to increase Nitric Oxide, there is preliminary evidence which indicates it can induce the secretion of Luteinizing Hormone (LH), which can in turn trigger Testosterone production. A 1995 study found that rats treated with Agmatine experienced increased LH secretion in a dose-dependent manner.
MAN Sports is no stranger to Agmatine (see NOOPump) and has elected to use an industry standard 500mg dose for the PepTest Bulk formula.
THE BOTTOM LINE
PepTest Bulk is yet another innovative pre-workout with a twist from the folks at MAN Sports. The cognitive/focus effects will be quite apparent to the average individual, and with a clinical dose of DAA per serving, users may experience increases in Testosterone as well. At just over $1 per serving, PepTest Bulk is fairly priced, and is worth a shot for anyone looking for a PWO/Test-Booster combination.
[expand title=”REFERENCES” tag=”h5″]
- Ando, S., et al. “Enhancement of learning capacity and cholinergic synaptic function by carnitine in aging rats.” Journal of neuroscience research 66.2 (2001): 266-271.
- Sano, Mary, et al. “Double-blind parallel design pilot study of acetyl levocarnitine in patients with Alzheimer’s disease.” Archives of neurology 49.11 (1992): 1137-1141.
- Rai, G., et al. “Double-blind, placebo controlled study of acetyl-l-carnitine in patients with Alzheimer’s dementia.” Current medical research and opinion 11.10 (1990): 638-647.
- Passeri, M., et al. “Acetyl-L-carnitine in the treatment of mildly demented elderly patients.” International journal of clinical pharmacology research 10.1-2 (1989): 75-79.
- Passeri, M., et al. “Mental impairment in aging: selection of patients, methods of evaluation and therapeutic possibilities of acetyl-L-carnitine.” International journal of clinical pharmacology research 8.5 (1987): 367-376.
- Bonavita, E. “Study of the efficacy and tolerability of L-acetylcarnitine therapy in the senile brain.” International journal of clinical pharmacology, therapy, and toxicology 24.9 (1986): 511-516.
- Iwu, Maurice M. Handbook of African medicinal plants. CRC press, 2014.
- Smith, C. “The effects of< i> Sceletium tortuosum in an< i> in vivo model of psychological stress.” Journal of ethnopharmacology 133.1 (2011): 31-36.
- Smith, Michael T., et al. “Psychoactive constituents of the genus< i> Sceletium NE Br. and other Mesembryanthemaceae: a review.” Journal of ethnopharmacology 50.3 (1996): 119-130
- Agharanya, Julius C., Raphael Alonso, and Richard J. Wurtman. “Changes in catecholamine excretion after short-term tyrosine ingestion in normally fed human subjects.” The American journal of clinical nutrition 34.1 (1981): 82-87.
- Fernstrom, John D., and Madelyn H. Fernstrom. “Tyrosine, phenylalanine, and catecholamine synthesis and function in the brain.” The Journal of nutrition137.6 (2007): 1539S-1547S
- Yeghiayan, Sylva K., et al. “Tyrosine improves behavioral and neurochemical deficits caused by cold exposure.” Physiology & behavior 72.3 (2001): 311-316
- Banderet, Louis E., and Harris R. Lieberman. “Treatment with tyrosine, a neurotransmitter precursor, reduces environmental stress in humans.” Brain research bulletin 22.4 (1989): 759-762.
- Shurtleff, David, et al. “Tyrosine reverses a cold-induced working memory deficit in humans.” Pharmacology Biochemistry and Behavior 47.4 (1994): 935-941.
- Lu, Hong, Xiaofeng Meng, and Chung S. Yang. “Enzymology of methylation of tea catechins and inhibition of catechol-O-methyltransferase by (−)-epigallocatechin gallate.” Drug metabolism and disposition 31.5 (2003): 572-579.
- Keränen, Tapani, et al. “Inhibition of soluble catechol-O-methyltransferase and single-dose pharmacokinetics after oral and intravenous administration of entacapone.” European journal of clinical pharmacology 46.2 (1994): 151-157.
- Ono, Yuka, et al. “Anti-obesity effect of< i> Nelumbo nucifera leaves extract in mice and rats.” Journal of Ethnopharmacology 106.2 (2006): 238-244.
- Nojima, Hiroshi, Mari Okazaki, and Ikuko Kimura. “Counter effects of higenamine and coryneine, components of aconite root, on acetylcholine release from motor nerve terminal in mice.” Journal of Asian natural products research 2.3 (2000): 195-203.
- Bai, Gang, et al. “Identification of higenamine in Radix Aconiti Lateralis Preparata as a beta2‐adrenergic receptor agonist1.” Acta Pharmacologica Sinica 29.10 (2008): 1187-1194.
- Barwell, C. J., et al. “Deamination of hordenine by monoamine oxidase and its action on vasa deferentia of the rat.” Journal of pharmacy and pharmacology41.6 (1989): 421-423.
- Falé, Pedro LV, et al. “Function of Plectranthus barbatus herbal tea as neuronal acetylcholinesterase inhibitor.” Food & function 2.2 (2011): 130-136.
- Porfírio, Sara, et al. “Antiacetylcholinesterase and antioxidant activities of< i> Plectranthus barbatus tea, after< i> in vitro gastrointestinal metabolism.” Food chemistry 122.1 (2010): 179-187.
- Morrissey, Jeremiah J., and Saulo Klahr. “Agmatine activation of nitric oxide synthase in endothelial cells.” Proceedings of the Association of American Physicians 109.1
- Abe, Kazuho, Yuzuru Abe, and Hiroshi Saito. “Agmatine suppresses nitric oxide production in microglia.” Brain research 872.1 (2000): 141-148.
- Mun, Chin Hee, et al. “Regulation of endothelial nitric oxide synthase by agmatine after transient global cerebral ischemia in rat brain.” Anatomy & cell biology 43.3 (2010): 230-240.
- D’Aniello, Gemma, et al. “d-Aspartate, a key element for the improvement of sperm quality.” Advances in Sexual Medicine 2 (2012): 45.
- Topo, Enza, et al. “The role and molecular mechanism of D-aspartic acid in the release and synthesis of LH and testosterone in humans and rats.” Reprod Biol Endocrinol 7.120 (2009): 6.
- Willoughby, Darryn S., and Brian Leutholtz. “d-Aspartic acid supplementation combined with 28 days of heavy resistance training has no effect on body composition, muscle strength, and serum hormones associated with the hypothalamo-pituitary-gonadal axis in resistance-trained men.” Nutrition Research 33.10 (2013): 803-810.
- D’Aniello, Autimo, Anna Di Cosmo, Carlo Di Cristo, Lucio Annunziato, Leonard Petrucelli, and George Fisher. “Involvement of D-Aspartic Acid in the Synthesis of Testosterone in Rat Testes.” Life Sciences 59.2 (1996): 97-104.
- Kalra, Satya P., et al. “Agmatine, a novel hypothalamic amine, stimulates pituitary luteinizing hormone release in vivo and hypothalamic luteinizing hormone-releasing hormone release in vitro.” Neuroscience letters 194.3 (1995): 165-168.