Athletix Ergonine Review

Athletix Ergonine

Ergonine is Athletix all-in-one pre-workout/recovery supplement. Athletix has elected to use a mostly transparent Supplement Facts panel, making it easy to see that the ingredients are, for the most part, dosed to clinical standards…


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Ergonine is Athletix’s all-in-one pre-workout/recovery supplement. Athletix has elected to use a mostly transparent Supplement Facts panel, making it easy to see that the ingredients are, for the most part, dosed to clinical standards…



Creatine is the most extensively studied ergogenic aid currently available, and by far one of the most effective at increasing both strength and muscle mass. Its primary mechanism of action is its ability to rapidly produce Adenosine Triphosphate (ATP) to support cellular energy, thereby directly increasing strength and power output.

Additionally, during high intensity exercise, Creatine is used for energy which tends to spare the glycogen that would normally be used. Since lactic acid is a by-product created when glucose is burned for energy, Creatine may also indirectly reduce lactic acid build-up which poses a secondary mechanism by which Creatine can potentially enhance performance.

Creatine Anhydrous is simply Creatine Monohydrate with the water molecule removed. This removal of the water molecule makes Creatine Anhydrous 100% Creatine by weight, as opposed to Creatine Monohydrate which is 88% Creatine.

This means that the 3g of Creatine Anhydrous present in one serving of Ergonine is equivalent to a slightly higher dose of Creatine Monohydrate.


Betaine (also known as Trimethylglycine) is the amino acid Glycine with the addition of three methyl groups attached. Betaine is alleged to increase power output and strength by increasing cellular swelling, a phenomenon well established with Creatine supplementation, which can drastically reduce the damaging effect of outside stimuli (such as exercise) on the working muscle. So far, Betaine has been investigated in several human studies, and has had some pretty encouraging results in most.

Feel free to read this article on Betaine, as it covers all the studies regarding performance enhancement.

Ergonine features a true clinical (2500mg) dose of Betaine per serving.


L-Alanyl L-Glutamine, as the name implies, is a dipeptide made up of Alanine and Glutamine. The idea behind L-Alanyl L-Glutamine is based on the notion that dipeptides are able to share the same transport into the cell, and can therefore be absorbed into the cell more effectively than two separate amino acids, which would require two separate transports.

A 2012 study showed better absorption of Glutamine (determined by plasma glutamine levels) when subjects ingested L-Alanyl L-Glutamine, than when they just ingested L-Glutamine alone.

A 2010 study, published in “The Journal of the International Society of Sports Nutrition”, found that L-Alanyl-L-Glutamine (as Sustamine) preserved hydration status in exercising male subjects which ultimately enhanced endurance.

Interestingly, a 2009 study from “Nutrition” found that the combination of free form Glutamine + Alanine was as effective as L-Alanyl-L-Glutamine at increasing Glutamine levels in mice, and that’s why Athletix has chosen to include Glutamine alongside L-Alanine.

Ergonine contains 2500mg of L-Alanine and L-Glutamine combined.


Carnitine, in various forms, has been studied extensively for a variety of implications. While results have been discouraging in the area of weight-loss, Carnitine is highly effective as a recovery agent.

Carnitine (as L-Tartrate) has been shown to significantly reduce markers of exercise-induced stress following exercise in healthy subjects and this has been replicated using 1-2g daily.

Although uncertain, the most likely mechanism of action is enhanced muscle oxygenation, as demonstrated in a 2008 study from “The Journal of Strength and Conditioning Research”.

Ergonine contains a highly effective 2000mg dose of L-Carnitine L-Tartrate per serving, precisely in-line with what has been used clinically.


A-Hydroxyisocaproic Acid (HICA) is a product of Leucine metabolism and is alleged to be an anti-catabolic agent.

A 2010 study found that athletes who consumed 1500mg of HICA per day for four weeks experienced an increase in lean body mass and a decrease in delayed onset muscle soreness (DOMS).

Ergonine contains the same 1500mg dose of HICA used in the above mentioned study.


Choline usually comes bearing the claim that it enhances cognition but Athletix makes it clear that the Choline Bitartrate in Ergonine is simply there to maintain adequate Choline stores, which can become depleted by rigorous exercise.

Athletix does reference a rather interesting study which found that Choline Bitartrate supplementation was able to reduce bodyweight while preserving strength in female martial arts athletes, but results like this need some replication before we can really draw conclusions there.

Ergonine contains an undisclosed amount of Choline Bitartrate.


Glycerol has become pretty popular over last couple years as a pump-inducing ingredient in pre-workout supplements. It’s mechanism of action is simple: Glycerol draws water into cells which can directly enhance what we all know as “The Pump”. Beyond that, Glycerol has been alleged to have actual performance enhancement implications as well.

A 1996 study, published in the “International Journal of Sports Medicine”, found that Glycerol supplementation prior to exercise increased endurance in cyclists. These findings were replicated in a 1999 study from the “European Journal of Applied Physiology and Occupational Physiology” in which pre-exercise Glycerol supplementation enhanced time performance (also in cyclists).

A 2003 study, published in the “Medicine and Science in Sports and Exercise”, found that, while post-exercise Glycerol supplementation prevented exercise-induced dehydration, this had no impact on performance measures (compared to placebo).

The research as a whole indicates that Glycerol can be an effective pump agent (due to water retention), but may only noticeably enhance performance (endurance not strength) during long-duration exercise when dehydration becomes a contributing factor to fatigue.

Ergonine contains HydroMax which is 65% Glycerol by weight.


Ferula Asafoetida contains Ferulic Acid which has been shown in to enhance exercise performance quite significantly in mice. We’re still waiting on the human research though.

A 2009 study, published in “Phytotherapy Research”, found that Ferula Asafoetida extract exhibited Acetylcholinesterase inhibiting properties. These results were replicated in mice in a later (2012) study from the “Indian Journal of Pharamcology”.


Ergonine is a well-formulated combination of some of the most extensively researched (and reliable) performance and recovery enhancing ingredients. Everything is effectively dosed, in-line with the current literature. Those looking for an effective non-stimulant pre-workout/post-workout combination should look no further than Ergonine.


[expand title=”REFERENCES” tag=”h5″]

  1. Jäger, Ralf, et al. “Analysis of the efficacy, safety, and regulatory status of novel forms of creatine.” Amino Acids 40.5 (2011): 1369-1383.
  2. Kraemer, William J., and Jeff S. Volek. “Creatine supplementation: its role in human performance.” Clinics in sports medicine 18.3 (1999): 651-666.
  3. Casey, Anna, and Paul L. Greenhaff. “Does dietary creatine supplementation play a role in skeletal muscle metabolism and performance?.” The American journal of clinical nutrition 72.2 (2000).
  4. Thompson, C. H., et al. “Effect of creatine on aerobic and anaerobic metabolism in skeletal muscle in swimmers.” British journal of sports medicine 30.3 (1996): 222-225.
  5. Kumar, Pradeep, V. K. Singh, and D. K. Singh. “Kinetics of enzyme inhibition by active molluscicidal agents ferulic acid, umbelliferone, eugenol and limonene in the nervous tissue of snail Lymnaea acuminata.” Phytotherapy Research 23.2 (2009): 172-177.
  6. Hoffman, Jay R., et al. “Effect of 15 days of betaine ingestion on concentric and eccentric force outputs during isokinetic exercise.” The Journal of Strength & Conditioning Research 25.8 (2011): 2235-2241
  7. i, Cheng, Masao Shinohara, John Kuhlenkamp, Christine Chan, and Neil Kaplowitz. “Mechanisms of Protection by the Betaine-homocysteine Methyltransferase/betaine System in HepG2 Cells and Primary Mouse Hepatocytes.” Hepatology 46.5 (2007): 1586-596.
  8. Trepanowski, John F., et al. “The effects of chronic betaine supplementation on exercise performance, skeletal muscle oxygen saturation and associated biochemical parameters in resistance trained men.” The Journal of Strength & Conditioning Research 25.12 (2011): 3461-3471.
  9. Hoffman, Jay R., et al. “Effect of betaine supplementation on power performance and fatigue.” Journal of the International Society of Sports Nutrition 6.1 (2009): 1-10.
  10. Cholewa, Jason M., et al. “Effects of betaine on body composition, performance, and homocysteine thiolactone.” Journal of the International Society of Sports Nutrition 10.1 (2013): 39.
  11. Lee, Elaine C., et al. “Ergogenic effects of betaine supplementation on strength and power performance.” J Int Soc Sports Nutr 7 (2010): 27.
  12. Vijayalakshmi, Shalini Adiga, et al. “Evaluation of the effect of Ferula asafoetida Linn. gum extract on learning and memory in Wistar rats.” Indian journal of pharmacology 44.1 (2012): 82.
  13. Anthony Troutner, Christina, and Mark Kern. “The effects of L-alanine supplementation on delayed onset muscle soreness and markers of muscle damage.” FASEB JOURNAL. Vol. 24. 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA: FEDERATION AMER SOC EXP BIOL, 2010.
  14. Cury-Boaventura, Maria Fernanda, et al. “Effects of exercise on leukocyte death: prevention by hydrolyzed whey protein enriched with glutamine dipeptide.” European journal of applied physiology 103.3 (2008): 289-294.
  15. Carvalho-Peixoto, Jacqueline, Robson Cardilo Alves, and Luiz-Claudio Cameron. “Glutamine and carbohydrate supplements reduce ammonemia increase during endurance field exercise.” Applied Physiology, Nutrition, and Metabolism 32.6 (2007): 1186-1190.
  16. Volek, Jeff S., et al. “L-Carnitine L-tartrate supplementation favorably affects markers of recovery from exercise stress.” American Journal of Physiology-Endocrinology and Metabolism 282.2 (2002): E474-E482.
  17. Bloomer, Richard J., Kelsey H. Fisher-Wellman, and Patrick S. Tucker. “Effect of oral acetyl L-carnitine arginate on resting and postprandial blood biomarkers in pre-diabetics.” Nutrition & metabolism 6.1 (2009): 25.
  18. Spiering, Barry A., et al. “Responses of criterion variables to different supplemental doses of L-carnitine L-tartrate.” The Journal of Strength & Conditioning Research 21.1 (2007): 259-264.
  19. Bloomer, Richard J., Lesley C. Tschume, and Webb A. Smith. “Glycine propionyl-L-carnitine modulates lipid peroxidation and nitric oxide in human subjects.” International journal for vitamin and nutrition research 79.3 (2009): 131-141.
  20. Sugino, Tomohiro, et al. “Effects of citric acid and L-carnitine on physical fatigue.” Journal of clinical biochemistry and nutrition 41.3 (2007): 224.
  21. Spiering, Barry A., et al. “Effects of L-carnitine L-tartrate supplementation on muscle oxygenation responses to resistance exercise.” The Journal of Strength & Conditioning Research 22.4 (2008): 1130-1135.
  22. Brass, Eric P. “Supplemental carnitine and exercise.” The American journal of clinical nutrition 72.2 (2000): 618s-623s.
  23. Mero, Antti A., et al. “Effects of alfa-hydroxy-isocaproic acid on body composition, DOMS and performance in athletes.” Journal of the International Society of Sports Nutrition 7.1 (2010)
  24. Magal, M. E. I. R., et al. “Comparison of glycerol and water hydration regimens on tennis-related performance.” Medicine and science in sports and exercise35.1 (2003): 150-156.
  25. Wingo, Jonathan E., et al. “Influence of a pre-exercise glycerol hydration beverage on performance and physiologic function during mountain-bike races in the heat.” Journal of athletic training 39.2 (2004): 169.
  26. Hitchins, S., et al. “Glycerol hyperhydration improves cycle time trial performance in hot humid conditions.” European journal of applied physiology and occupational physiology 80.5 (1999): 494-501
  27. Feduccia Tayebati, Seyed Khosrow, et al. “Effect of choline-containing phospholipids on brain cholinergic transporters in the rat.” Journal of the neurological sciences302.1 (2011): 49-57.
  28. Tomassoni, Daniele, et al. “Effects of cholinergic enhancing drugs on cholinergic transporters in the brain and peripheral blood lymphocytes of spontaneously hypertensive rats.” Current Alzheimer Research 9.1 (2012): 120-127.
  29. Gimenez, Rosa, Josep Raich, and Juan Aguilar. “Changes in brain striatum dopamine and acetylcholine receptors induced by chronic CDP‐choline treatment of aging mice.” British journal of pharmacology 104.3 (1991): 575-578.
  30. Wallace, Julie MW, et al. “Choline supplementation and measures of choline and betaine status: a randomised, controlled trial in postmenopausal women.” British Journal of Nutrition 108.07 (2012): 1264-1271.
  31. Penry, Jason T., and Melinda M. Manore. “Choline: an important micronutrient for maximal endurance-exercise performance?.” International journal of sport nutrition and exercise metabolism 18.2 (2008): 191.
  32. Elsawy, Gehan, Osama Abdelrahman, and Amr Hamza. “Effect of choline supplementation on rapid weight loss and biochemical variables among female Taekwondo and Judo athletes.” Journal of human kinetics 40.1 (2014): 77-82.
  33. Cruzat, Vinicius Fernandes, and Julio Tirapegui. “Effects of oral supplementation with glutamine and alanyl-glutamine on glutamine, glutamate, and glutathione status in trained rats and subjected to long-duration exercise.”Nutrition 25.4 (2009): 428-435.
  34. Hoffman, Jay R., et al. “Examination of the efficacy of acute L-alanyl-L-glutamine ingestion during hydration stress in endurance exercise.” Journal of the International Society of Sports Nutrition 7.8 (2010).
  35. Cruzat, Vinicius Fernandes, and Julio Tirapegui. “Effects of oral supplementation with glutamine and alanyl-glutamine on glutamine, glutamate, and glutathione status in trained rats and subjected to long-duration exercise.”Nutrition 25.4 (2009): 428-435.
  36. You, Yanghee, et al. “Chronic effect of ferulic acid from Pseudosasa japonica leaves on enhancing exercise activity in mice.” Phytotherapy Research 24.10 (2010): 1508-1513.
  37. You, Yanghee, et al. “Stimulatory effects of ferulic acid on endurance exercise capacity in mice.” Bioscience, biotechnology, and biochemistry 73.6 (2009): 1392-1397.

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