Isa-Test DA3 Review

Isa-Test DA3

Isa-Test DA3 is iSatori’s Testosterone booster which contains, among other things, a clinical dose of D-Aspartic Acid…


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Isa-Test DA3 is iSatori’s Testosterone booster which contains, among other things, a clinical dose of D-Aspartic Acid…[SKip to the Bottom Line]


D-Aspartic Acid acts as a precursor to N-Methyl-D-aspartic acid (NMDA), which takes on a similar role to the excitatory neurotransmitter Glutamic Acid. In rats, D-Aspartic acid supplementation has been shown to significantly increase Testosterone. Out of the three human studies done specifically to test the effect of D-Aspartic Acid on Testosterone, two have shown a significant increase in Testosterone levels and one has failed to do so.

A 2012 study from “Advances in Sexual Medicine”, the subjects of which were infertile men (low testosterone) found that 2.66g of D-Aspartic Acid was able to significantly increase Testosterone levels when measured after 90 days of supplementation. These results were in-line with those of an earlier (2009) study in which D-Aspartic Acid supplementation raised Testosterone by 42% after 12 days in healthy men. However, a 2013 study published in “Nutrition Research” found that athletes who supplemented with D-Aspartic Acid for 28 days showed no difference in testosterone levels.

The researchers in the failed study noted abnormally high levels of D-aspartate oxidase, the enzyme which degrades D-Aspartic Acid, indicating that prolonged supplementation in individuals with healthy Testosterone levels may cause “negative feedback” which reduces the effects. Ultimately, D-Aspartic acid is an effective short-term Test-booster for healthy individuals, but the effects may fade after a few weeks of supplementation. Isa-Test DA3 contains 3000mg of D-Aspartic Acid, in-line with the above mentioned positive studies.


Ashwagandha is a popular Ayurvedic herb with a relatively wide variety of health implications, ranging from cognitive support to anti-cancer effects. In the context of Isa-Test DA3, iSatori is primarily concerned with Testosterone optimization.

A 2010 study, published in Fertility and Sterility”, found that Ashwagandha (5g basic root powder) was able to restore Testosterone levels in infertile men, though subjects did not experience spikes beyond the normal range. These findings were replicated in a 2011 study in which it was also noted that the effects were more apparent in stressed men (Ashwagandha is a known adaptogen).

Unfortunately, while the above mentioned studies certainly indicate that Ashwagandha is effective for restoring Testosterone in deficient individuals, there is no evidence to suggest it can boost Testosterone in non-deficient individuals.


Cordyceps is a mushroom with a relatively well-documented history of use in Traditional Chinese Medicine for a variety of ailments. However, as with Ashwagandha, iSatori has included Cordyceps in the Isa-Test DA3 formula based on preliminary evidence to suggest a role in regulating Testosterone production.

A 2003 study published in “Life Sciences” showed a statistically significant (but not overwhelming) increase in Testosterone in mice fed a Cordyceps-augmented diet. Similar findings were obtained in a 2008 study, published in “The American Journal of Chinese Medicine”, at a dose of around .2mg/kg (again in rats).

The mechanism of action by which Cordyceps may increase Testosterone is via upregulating of the steroidogenic acute regulatory protein (StAR) enzyme which is responsible for transporting cholesterol to the mitochondria for Testosterone (and other hormone) synthesis. Unfortunately, there is no human data upon which to base conclusions about the effects of Cordyceps on Testosterone levels, though the preliminary evidence certainly warrants such research.


Trigonella Foenum Greacum (also known as Fenugreek) has gained serious traction in the supplement industry as a libido enhancer and alleged Testosterone-booster. However, a 2009 study, published in the “International Journal of Exercise Science”, found that Fenugreek supplementation had no influence on Testosterone (or any other hormone). A similar failure was noted in a 2011 double-blind, placebo controlled study in which 6 weeks of supplementation with a Fenugreek derived extract (Testofen) led to scoring 25% higher on a libido test (sexual arousal and orgasm in particular) than the placebo group, but with no increase in Testosterone levels, meaning that the mechanism of action was not an increase in Testosterone.

While two studies have failed to show any Testosterone-boosting effect of Fenugreek supplementation, one 2010 study, published in “The International Journal of Sports Nutrition”, found that supplementation with 500 mg of Fenugreek extract (Testofen again) resulted in a significant increase in free-Testosterone levels.

Ultimately, the results are mixed, with two studies failing and one study showing increased Testosterone. More research is needed to clear up this discrepancy, but for now it appears Fenugreek is only reliable as a libido enhancer, not a Testosterone booster. The exact dose of Fenugreek in the Isa-Test DA3 formula is undisclosed, but even at 500mg the effects are unreliable (so it doesn’t really matter much).


Eurycoma Longifolia, also known as Tongkat Ali has been shown, in various studies, to increase Testosterone in male rats, but the only human studies that exist have tested the effects of Tongkat Ali in infertile men, not healthy men.

A 2010 study published in the “Asian Journal of Andrology” found that supplementation with 200mg of an extract of Eurycoma Longifolia significantly improved various indications of male fertility (in humans), though the mechanism of action was unknown. A 2012 study published in “Andrologia: Volume 44” (the same researchers from the above mentioned human study) found that men suffering from Hypogonadism (diminishing functionality of the gonads) who were treated with a 200 mg daily dose of Eurycoma longifolia extract reached normal Testosterone levels after a 30 day period. To be fair, at the start of the study about 35% of the men were showing normal Testosterone levels, and at the end about 90% showed normal levels. Still, 35% to 90% is clearly statistically significant.

Isa-Test DA3 contains an unknown quantity of Tongkat Ali, based on the label we estimate it contains anywhere from 50-150mg.


Epimedium (also known as Horny Goat Weed) is generally used as an aphrodisiac and libido enhancer, but does possess mechanisms by which it may boost Testosterone. These effects are primarily attributed to the active compound, Icariin.

A 2006 study, published in the “Asian Journal of Andrology”, found that Icariin (80mg/kg) was able to increase Testosterone roughly three times as much as the control group in chemically castrated mice. However, the dose used in this particular study would be roughly equivalent to a 200lb human consuming 1150mg pure Icariin daily. That being said, Icariin has demonstrated varying degrees of efficacy at varying doses, so it is possible that there is a slight influence at the dose present in Isa-Test DA3. At the very least, Icariin supplementation is generally associated with enhanced libido and sexual well-being (providing the illusion of increased Testosterone without the strength gains).

Isa-Test DA3 contains an undisclosed quantiy of Epimedium, but its safe bet that the dose is far less than what has proved effective in mice. Still, it may certainly lead to enhanced libido.


Isa-Test DA3 contains several of the usual alleged Test-boosters, most of which have atleast preliminary evidence regarding Testosterone-optimization. D-Aspartic Acid is the only ingredient which has been demonstrated to outright boost Testosterone in healthy individuals, with most of the others demonstrating the ability to restore/optimize levels in deficient individuals. For this reason, whether or not Isa-Test DA3 raises Testosterone likely depends on individual Testosterone status prior to supplementation. It’s also important to note that the formula contains several aphrodisiacs and libido enhancers that may provide the illusion of increased Testosterone but without the strength gains that usually accompany higher Test levels.

[expand title=”REFERENCES” tag=”h5″]

  1. D’Aniello, Autimo, Anna Di Cosmo, Carlo Di Cristo, Lucio Annunziato, Leonard Petrucelli, and George Fisher. “Involvement of D-Aspartic Acid in the Synthesis of Testosterone in Rat Testes.” Life Sciences 59.2 (1996): 97-104.
  2. D’Aniello, Gemma, et al. “d-Aspartate, a key element for the improvement of sperm quality.” Advances in Sexual Medicine 2 (2012): 45.
  3. Topo, Enza, et al. “The role and molecular mechanism of D-aspartic acid in the release and synthesis of LH and testosterone in humans and rats.” Reprod Biol Endocrinol 7.120 (2009): 6.
  4. Willoughby, Darryn S., and Brian Leutholtz. “d-Aspartic acid supplementation combined with 28 days of heavy resistance training has no effect on body composition, muscle strength, and serum hormones associated with the hypothalamo-pituitary-gonadal axis in resistance-trained men.” Nutrition Research 33.10 (2013): 803-810.
  5. Bushey, Brandon, et al. “Fenugreek Extract Supplementation Has No effect on the Hormonal Profile of Resitance-Trained Males.” International Journal of Exercise Science: Conference Proceedings. Vol. 2. No. 1. 2009.
  6. Steels E, Rao A, Vitetta L. Physiological Aspects of Male Libido Enhanced by Standardized Trigonella foenum-graecum Extract and Mineral Formulation. Phytother Res. (2011)
  7. Tambi, M. I. B. M., M. K. Imran, and R. R. Henkel. “Standardised water‐soluble extract of Eurycoma longifolia, Tongkat ali, as testosterone booster for managing men with late‐onset hypogonadism?.” Andrologia 44.s1 (2012): 226-230.
  8. Ang, H. H., S. Ikeda, and E. K. Gan. “Evaluation of the potency activity of aphrodisiac in Eurycoma longifolia Jack.” Phytotherapy Research 15.5 (2001): 435-436.
  9. Zanoli, P., et al. “Influence of< i> Eurycoma longifolia on the copulatory activity of sexually sluggish and impotent male rats.” Journal of ethnopharmacology 126.2 (2009): 308-313.
  10. Ang, H. H., S. Ikeda, and E. K. Gan. “Evaluation of the potency activity of aphrodisiac in Eurycoma longifolia Jack.” Phytotherapy Research 15.5 (2001): 435-436.
  11. Tambi, Mohd Ismail Bin Mohd, and M. Kamarul Imran. “Eurycoma longifolia Jack in managing idiopathic male infertility.” Asian journal of andrology 12.3 (2010): 376-380.
  12. Bhat, Rajeev, and A. A. Karim. “Tongkat Ali (< i> Eurycoma longifolia Jack): A review on its ethnobotany and pharmacological importance.”Fitoterapia 81.7 (2010): 669-679.
  13. Zhang, Zhen‐Bao, and Qing‐Tao Yang. “The testosterone mimetic properties of icariin.” Asian journal of andrology 8.5 (2006): 601-605.
  14. Chang, Ying, et al. “Effect of Cordyceps militaris supplementation on sperm production, sperm motility and hormones in Sprague-Dawley rats.” The American journal of Chinese medicine 36.05 (2008): 849-859.
  15. Hsu, Chih-Chao, et al. “In vivo and in vitro stimulatory effects of< i> Cordyceps sinensis on testosterone production in mouse Leydig cells.” Life sciences73.16 (2003): 2127-2136.
  16. Kiasalari, Zahra, Mohsen Khalili, and Mahbobeh Aghaei. “Effect of withania somnifera on levels of sex hormones in the diabetic male rats.” Iranian Journal of Reproductive Medicine 7.4 (2009): 163-168.
  17. Ahmad, Mohammad Kaleem, et al. “< i> Withania somnifera improves semen quality by regulating reproductive hormone levels and oxidative stress in seminal plasma of infertile males.” Fertility and sterility 94.3 (2010): 989-996.
  18. Mahdi, Abbas Ali, et al. “Withania somnifera improves semen quality in estress-related male fertility.” Evidence-Based Complementary and Alternative Medicine2011 (2011).

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